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Automated Cell Counter for cGMP
- 21 CFR part 11 compliance
- Stem cell, CAR-T cell, CAR-NK cell, Adipose-derived stem cell
- Accurate cell counting and viability
- Automated image analysis
- 4TB extra storage for data backup and recovery
Various Applications Cell Counter for cGMP
ADAM™ CellT complies with 21 CFR part 11. When 21 CFR part 11 mode is activated, data cannot be modified by any user.
Every action is recorded in an audit trail including the date, time and specific details of the action.
ADAM™ calibration beads allow quick diagnosis when problems occur, which ensures accuracy of the instrument.
PI-Staining Method
After the samples are stained with fluorescent dye, propidium iodide, which intercalates DNA to stain the nucleus of target cells,
ADAM™ CellT takes fluorescent images automatically.
The obtained images are processed by image analysis software integrated inside the system
21 CFR Part 11 Compliance
ADAM™ CellT complies 21 CFR part 11 which is a regulation about electronic records and signatures for use in cGMP facilities.
When 21 CFR Part 11 mode is activated, data cannot be modified by any user.
Every action of users is recorded in an audit trail which includes the date, time, and specific details of the action. Electronic signatures based upon biometrics are designed to ensure that they cannot be reused by, or assigned to, anyone else.
User Management
Access level and rights of users
21 CFR Part 11 consists of limiting system access to authorized individuals.
Users are able to control access within software. It is designated to separate 3 different groups - Admin, Supervisor and User.
QC Platform for producing CAR-T cell
It is easy to monitor all different steps of the purification, expansion, and formulation of CAR-T cells using the ADAM™ to ensure precise and reliable results. It can be used for cGMP, process control and quality control of CAR-T cell.
Performance Test from Isolated T cells
Figure 1 shows accuracy of ADAM™-CellT which is a a correlation of Tcell-counting between flow cytometry and ADAM™ in Isolated T cells. Figure 2 shows repeatability when the concentration of of isolated T cells is low, medium and high.
Accuracy of system
Validation of ADAM™-CellT
ADAM™ calibration beads allow quick diagnosis when problems occur, which ensures accuracy of the instrument.
Installation Qualification (IQ), Operational Qualification (OQ) and Performance Qualification (PQ) are designed to validate performance of the instrument.
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Single-Cell Analyses Identify Brain Mural Cells Expressing CD19 as Potential Off-Tumor Targets for CAR-T Immunotherapies, Cell, 183 (1), pp. 126-142.e17, October, 01, 2020. Abstract CD19-directed immunotherapies are clinically effective for treating B cell malignancies but also cause a high incidence of neurotoxicity. A subset of patients treated with chimeric antigen receptor (CAR) T cells or bispecific T cell engager (BiTE) antibodies display severe neurotoxicity, including fatal cerebral edema associated with T cell infiltration into the brain. |
| Expression of the transcription factor PU.1 induces the generation of microglia-like cells in human cortical organoids, Nature Communications, 13, 430 (2022) |
| Heterozygous frameshift variants in HNRNPA2B1 cause early-onset oculopharyngeal muscular dystrophy, Nature Communications, 13, 2306 (2022) |
| A Fully-Human Antibody Specifically Targeting a Membrane-Bound Fragment of CADM1 Potentiates the T Cell-Mediated Death of Human Small-Cell Lung Cancer Cells, International Journal of Molecular Sciences, 23.13 (2022) |
| Generation and Next-Generation Sequencing-Based Characterization of a Large Human Combinatorial Antibody Library, International Journal of Molecular Sciences, 2023 |
| A murine model of hnRNPH2-related neurodevelopmental disorder reveals a mechanism for genetic compensation by Hnrnph1. Journal of Clinical Investigation, 2023 (Preprint@bioRxiv: https://doi.org/10.1101/2022.03.17.484791) |
| A novel bispecific antibody dual-targeting approach for enhanced neutralization against fast-evolving SARS-CoV-2 variants. Frontiers in Immunology, 2023 |
| Identification of small molecule inhibitors of G3BP-driven stress granule formation. Journal of Cell Biology, 2024 |
| Empowering SARS-CoV-2 variant neutralization with a bifunctional antibody engineered with tandem heptad repeat 2 peptide. Journal of Medical Virology, 2024 |
| A dual-targeting approach using a human bispecific antibody against the receptor-binding domain of the Middle East Respiratory Syndrome Coronavirus. Virus Research, 2024 |
| Development of a novel CD3ε-specific human antibody for selective modulation of T cell activation. International Journal of Biological Macromolecules, 2025 |
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Product name / Cat. No.
ADAM™ CellT / ADAM™ CellT
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Loading Volume
13 µL/test (4 ch)
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Measuring Volume
3.4 µL/test (4ch)
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Measuring range
5 X 10E4–4 X 10E6 cells/mL (PI)
5 X 10E4–2 X 10E7 cells/mL (AO/PI) -
Focus
Auto-focusing
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LED
4W Green LED
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Weight
7.0 Kg
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Size
277 X 276 X 270 mm
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Analysis time
< 25 sec/test (4 ch)
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Dyes
PI (Propidium Iodide)
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Connectivity
WiFi (USB Dongle)
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Cell type
Cell line (clumpy cell, single cell), PMBCs, Adipose Stem cell, Total white blood cell, Primary cells
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Extra storage for data backup and recovery
4TB
Related downloads Total 3
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ADAM-CellT_brochure_NanoEntek
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ADAM-CellT_Manual_NanoEntek
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ADAM-CellT_performance_evaluation_of_CAR-T_cell_NanoEntek
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LS PRODUCT / ADAM™ CellT
Bead calibration results are out of range.
1. Please try vortexing of the bead for a minute, or shake the bead bottle.2. Use bead from the new bead bottle. -
LS PRODUCT / ADAM™ CellT
I see a good number of debris in the images taken.
1. Check if there are no scratches outside or no debris inside the r-Slide.2. If you still see debris when using a new control sample, the control sample may be defective. Please contact your control sample manufacturer.
ExTransfection™ Protocol Library
ExTransfection™ provides open protocols that make it easy for users to set up and run experiments. Its 24-well optimization method helps users to optimize transfection protocols easily and quickly.
ExTransfection™ also offers a pre-programmed optimization protocol that helps an easy and quick optimization of electrical parameters for both adherent and suspended cells.
