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Disposables for ADAM-MC Series
In contrast, cells with damaged membranes or with inactive metabolism are unable to prevent PI from entering themselves.
As a result, only the nuclei of non-viable cells are stained.
AccuStain Solution T for the total cell counting is composed of fluorescent dye (PI) and lysis solution.
AccuStain Solution N for the non-viable cell counting is composed of fluorescent dye and PBS.
In order to measure the total concentration of cells, the plasma membranes of all cells must be disrupted to stain all the Nuclei with PI.
The process of disrupting and staining is achieved by treatment with AccuStain Solution T.
ADAM™-MC,MC2 and CellT
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Product name / Cat. No.
AccuChip Kit / AD4K–200
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Measuring range
5X10E4–4X10E6 cells/mL
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Staining method
PI (Propidium lodide)
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Sample loading volume
13 μL
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Contents
–AccuChip: 200 slides (4 channel)
–AccuStain Solution (T): 12.5 ml x 2 bottles
–AccuStain Solution (N): 12.5 ml x 1 bottle -
Quantity
200 slides / kit
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ExTransfection™ provides open protocols that make it easy for users to set up and run experiments. Its 24-well optimization method helps users to optimize transfection protocols easily and quickly.
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