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The Most Accurate Cell Counter
- Less than 25 sec to get results
- Cell therapy quality control
- Cells countable with ADAM MC2: stem cell / CAR-T cell / CAR-NK cell / Adipose-derived stem cell
- Automated image analysis
- Accurate result, sensitve CMOS detection, and precise automatic stage
A Fast & Accurate Nucleus Cell Counter
ADAM™ stands for Advanced Detection and Accurate Measurement.
- Cell therapy quality control
- Cells countable with ADAM™ MC2: stem cell/ CAR-T cell/ CAR-NK cell/ Adipose-derived stem cell
- Automated image analysis
- Accurate result with PI staining method
- Sensitive CMOS detection, and precise automatic stage
Principle of Viability Measurement (AO, PI-Staining Method)
There are two methods of viability measurement. After the samples are stained with fluorescent dye, propidium iodide(PI) or acridine orange (AO), which intercalates DNA to stain the nucleus of target cells, ADAM™ MC2 takes fluorescent images automatically.
The obtained images are processed by image analysis software integrated inside the system.
Counting Aggregated and Irregular-Shaped Cells
ADAM™ MC2 provides accurate and reliable results because it counts aggregated and irregular-shaped cells.
In the image below, the images on the right side indicate cells that have been counted by ADAM™-MC2.
- Accurate count based on cell size and shape
- Count aggregated cells individually
- Debris is excluded from results
Cell Therapeutic Applications
ADAM™ MC2 can be used as a device to monitor and QC the cell numbers and viability
in the process of manufacturing cells (CAR-T cells, stem cells, etc.) for cell therapy.
In addition, It is possible to use ADAM™ MC2 depending on the cell types (Whole blood cell, PBMCs, etc.)
that need to be monitored during the manufacturing of cell therapy products.
Accuracy & Reproducibility
The graph on the left shows correlation of total cell counting between hemocytometer and ADAM™ MC2 using SH-SY5Y cells.
The graph on the right shows that samples with low, medium and high concentration of cells were counted with ADAM™ MC2.
The repeatability at each level of cell concentration is high.
Comparison of Cell Viability
Comparison of cell viability between ADAM™ MC2, flow cytometry, and manual counting
SH-SY5Y, Jurkat, HeLa cells were treated with 100, 300 μM H2O2 for 3 hours,
then analyzed by ADAM™ MC2, flow cytometry, and manual counting.
Reference paper of using ADAM™ MC2
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Title |
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Hybrid SMART spheroids to enhance stem cell therapy for CNS injuries. Science advances, 2021 Link: https://doi.org/10.1126/sciadv.abj2281 |
| Plasma complement C7 as a target in non-small cell lung cancer patients to implement 3P medicine strategies. EPMA Journal, 2021 Link: https://doi.org/10.1007/s13167-021-00266-x
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Adoptive immunotherapy with transient anti-CD4 treatment enhances anti-tumor response by increasing IL-18Rαhi CD8+ T cell, Nature Communications, 2021 Link: https://doi.org/10.1038/s41467-021-25559-7 |
| IL7-Fc Enhances the Efficacy of Adoptive T Cell Therapy under Lymphopenic Conditions in a Murine Melanoma Model, Cells, 2021 Link: https://doi.org/10.3390/cells10082018 |
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TDP-43 and PINK1 mediate CHCHD10 S59L mutation–induced defects in Drosophila and in vitro. Nature Communications, 2021 Link: https://doi.org/10.1038/s41467-021-22145-9 |
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Polymorphic Region-Specific Antibody for Evaluation of Affinity-Associated Profile of Chimeric Antigen Receptor, Molecular Therapy Oncolytics, 2020 Link: https://www.sciencedirect.com/science/article/pii/S2372770520300541 |
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Chronic activation of 4-1BB signaling induces granuloma development in tumor-draining lymph nodes that is detrimental to subsequent CD8+ T cell responses, Cellular & Molecular Immunology, 2020 Link: https://doi.org/10.1038/s41423-020-00533-3 |
| Differential physical interactions of wild-type and S59L mutant CHCHD10 with mitochondrial proteases, F1000Research, 2024 Link: https://doi.org/10.12688/f1000research.151962.1 |
| FDA-approved PDE4 inhibitors alleviate the dominant toxicity of ALS–FTD-associated CHCHD10S59L by reducing the PINK1/Parkin pathway, Research Square, 2024 Link: https://doi.org/10.21203/rs.3.rs-4870330/v1
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Product name / Cat. No.
ADAM–MC2 / ADAM–MC2
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Loading volume
13 µL/test (4 ch)
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Measuring volume
3.4 µL/test (4ch)
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Measuring range
5 X 10E4–4 X 10E6 cells/mL(PI)
5 X 10E4–2 X 10E7 cells/mL (AO/PI) -
Focus
Auto–focusing
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LED
4W Green LED
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Weight
7.0 kg
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Size (LxWxH)
277 x 276 x 270 mm
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Analysis time
< 25 sec/test (4 ch)
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Dyes
PI (Propidium Iodide) or AO (Acridine Orange)
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Connectivity
WIFI (USB Dongle)
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Cell type
Cell line (clumpy cell, single cell), PBMCs, Adipose Stem cell, Total white blood cell, Primary cells, SVF–derived cells
Related downloads Total 2
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ADAM-MC2_Brochure_NanoEntek
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ADAM-MC2_User_manual_NanoEntek
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