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ADAM™ CellT Plus

Most Accurate Fluorescence Cell Counter

ADAM™ CellT Plus is an advanced automated cell counter featuring bright field and two fluorescence channels (AO/DAPI), ensuring highly accurate cell counting. It offers a comprehensive solution tailored to meet the requirements of 21 CFR Part 11 within cGMP production environments. 

  


KEY FEATURES & BENEFITS 

- FAST results in 10 sec per test

- LESS volume of sample (15 μL) required 

- LARGER measuring volume

- COMPLIANT with 21 CFR part 11

- ACCURATE fluorescence cell count & viability 

- SAVING cost with 4 channel slide 





 


ADAM™ CellT Plus 
ADAM™ CellT Plus is an automated cell counter that is available in cGMP production environment which provides a comprehensive solution to comply with the requirements of 21 CFR Part 11. 

 

Accurate | Fast | Convenient | Compliant


Basic principle of counting
Total cell number and cell viability (AO/DAPI staining)

 

ADAM™ CellT Plus is an automated fluorescence cell counter that utilizes 

Acridine Orange (AO) and DAPI (4′,6-diamidino-2-phenylindole) to stain total and dead cells, respectively. 


AO/DAPI stain nucleated cells only, effectively excluding interferences such as debris and red blood cells in samples 

with complex backgrounds, thus providing accurate cell counts and viability analysis. 


Moreover, ADAM™ CellT Plus is equipped with bright field imaging capabilities, 

expanding its utility across a wide range of applications in both industrial and academic settings.  












Large Measuring Volume | Expanded Field of Views

 

ADAM CellT Plus measures larger volume of sample, and captures more image frames each channel. 

Leveraging this capability, ADAM CellT Plus provides superior repeatability and accuracy 

compared to other cell counting products in the market.  

                               
          
    







Cell Size Measurement & Cell Aggregation Rate

 

ADAM™ CellT Plus not only measures the number of total cells, viable cells, non-viable cells, and viability results.

In addition, they analyzes the cell size and cell aggregation ratio as well.







Accuracy & Precision



Accuracy & Repeatability
Correlation of PBMCs total counting between flow cytometry, manual count and ADAM™ CellT Plus.

 




  

 

 

Data were compared between flow cytometer and ADAM™ MC Plus & CellT Plus results in 20 different PBMC concentration sample.

 

Data were compared between manual count and ADAM™ MC Plus & cellT Plus results in 20 different PBMC concentration samples.







Linearity & Reproducibility

 

 

 

A high-concentration (1.8E+07 cells/mL) of PBMCs was diluted and a dilution series was counted by ADAM MC Plus & CellT Plus. 

It shows excellent dilution linearity and reproducibility.

 

Sample with low, medium and high concentration of PBMCs were counted with 

three ADAM MC Plus & CellT Plus. 





A Wide Range of Applications



Cell Therapeutic Applications
ADAM™ CellT Plus is ideal cell counter for the monitoring and QC of the cell numbers and viability in the process of manufacturing a variety of cells (CAR-T cells, stem cells, etc.) for Cell Therapy under cGMP production environment.




 




21 CFR Part 11 Compliance



User Management & Access Control


There are three designated user groups: Admin, Supervisor, and User. 

Admin have the highest level of privileges that can set account level to the users or release the accessibility to the data.

 

 



 







Record & Audit Trail 


Every action of users is recorded in an audit trail which includes the date, time, and specific details of the action. All action logs are recorded to ensure data integrity and traceability of data management. 

Electronic Signature 


All reports may be digitally signed. The electronic signature information includes the name and user ID of the signer, the date and time of signature execution and meaning (e.g. review or approval) of the signature.

 



 

 



 

 










Validation of ADAM™ CellT Plus

 

ADAM™ calibration bead and QC slide (optional) allow quick diagnosis when problems occur, which ensures accuracy of the instrument.

Installation Qualification (IQ), Operational Qualification (OQ) and Performance Qualification (PQ) are designed to validate performance of the instrument. 







NanoEntek blog is now open!

Want to learn more about cell counting and cell therapy?

Visit to find out more!

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FOR RESEARCH USE ONLY.
This product is not approved for diagnostic or therapeutic use.
 

 

 

  • Cat no.

    ADAM–CellT Plus

  • Focus

    Auto–focusing

  • Objective lens

    4 X

  • Measuring range

    5 X 10E4–2 X 10E7 cells/mL (Detectable)
    4 X 10E5–1 X 10E6 cells/mL (Optimal)

  • Size and weight

    277 x 276 x 270mm, 7kg

  • Loading volume

    15 uL / test

  • Channel

    Bright field / 2 Fluorescence (AO & DAPI)

  • Staining method

    Acridine orange / DAPI solution

  • Measuring volume

    3.2 µL / test

  • Measuring time

    Accuracy mode (AO/DP, Default): 17 sec/test
    Fast mode (AO/DP): 10 sec/test

ExTransfection™ Protocol Library

ExTransfection™ provides open protocols that make it easy for users to set up and run experiments. Its 24-well optimization method helps users to optimize transfection protocols easily and quickly.

ExTransfection™ also offers a pre-programmed optimization protocol that helps an easy and quick optimization of electrical parameters for both adherent and suspended cells.