NanoEntek

A useful CD34 counter for stem cell therapy, ADAMII-CD34

Stem cell therapy, which is seen as a cure for various diseases, is expected to be worth about 3.38 billion USD in 2023. As the demand increase for cell therapies and research actively progress, the accurate total cell number and viability have become the main factors as well as reproducibility.

NanoEntek's ADAMII-CD34 provides reasonable device price and inspection costs for stem cell transplantation and treatment compared to competing equipment. There are advantages to using simple training without any experts, and it reduces the maintenance cost since the kits are disposable

"What is the Stem cell therapy?"

Cell therapy is the drug that induces regeneration for restoring damaged or diseased cells by using the living cell. It can be used for new drug development, toxicology testing, bio-marker and so on. Cell therapies can be divided into three parts; tissue cell therapy, immune cell therapy and stem cell therapy. We will specifically focus on the stem cell therapy.

Biomedicine can be divided into cell therapy and gene therapy, and cell therapy can be subdivided into tissue-cell therapy, immuno-cell therapy and stem-cell therapy. Also, gene therapy can be subdivided into immune cell gene therapy and stem cell gene therapy [1].

Treatment through embryonic stem cells

Macula, where most of the visual cells are concentrated, is in charge of an important role in vision. Macular degeneration is a disease that degrades the function of the macular and causes blindness. The patient who have macular degeneration and Stargardt disease with no cure got to hear good news. Roski eye institute from the University of Southern California transplanted retinal pigment epithelium cells (RPE cells) from embryonic stem cells into four patients with macular degeneration. As a result, all of them have cured from the disease, sme of them have improved their eyesight. [2]

In addition, clinical results were announced by May 2015 that improved vision through embryonic stem cell therapy.

Where can I use the stem cell therapy?

The stem cell is the source of all cells. It is a cell that is undifferentiated, and it can be self-replicating and be differentiated. The damage organs or tissues can be treated using the differentiation function because they can be divided and duplicated indefinitely.

Stem cells can be classified into an adult stem cells (AS Cell), induced pluripotent stem cells(iPS cells) and embryonic stem cells (ES cells).

Stem cell therapy that have the ability to differentiate and multiply into various cells. Many studies have been conducted and manufactured to apply stem cell therapy to various diseases according to technological advances. In addition, the majority of developed countries provide policy support as an one of the medical industries. The stem cell is used as a criterion by biomarker to monitor the effectiveness of post-treatments, and it can be used as cell treatments for incurable diseases.

Stem cells can be injected into patients through differentiation and proliferation. It can replace damaged cells, and tissue functions can be developed as cell therapy. In addition, the stem cell can contribute to the efficiency of new drug development by finding new drug candidates and examining their efficacy. Stem cells, which have shown effects such as treating burn patients, cardiovascular diseases, treating muscle disease, recovering eye sight, and Alzheimer;s disease, are also expected to treat diseases such as, spinal cord injury, dementia, Crohn's disease, Parkinson's disease, Lou Gehrig's disease, and multiple sclerosis.

Among stem cells , Hematopoietic stem cell (HSC) produces red blood cells (RBC, Erythocyte), white blood cells (WBC, Leukocyte), platelet and immune cell such as T-lymphocytes, B-lymphocytes. The hematopoietic stem cell is used to treat aplastic anemia, acute leukemia, and solid cancer. Hematopoietic stem cell transplantation (HSCT) is rapidly increasing worldwide.

Why choose NanoEntek?

Comparison ADAMII-CD34 with flow cytometry

The traditional method used for cell counting is called flow cytometry represented by a fluorescence-activated cell sorter.

The flow cytometry is expensive, and it is also inconvenient due to the size of equipment and incidental factors. Additional work for management before and after using the equipment also takes a lot of reagents, time and effort. When applying a strong electric field to cell separation, there are disadvantages of high damage to the cell and the need of an expert in the operation and management of the equipment.

Features of ADAMII-CD34

In what ways ADAMII-CD34 can be used

The ADAMII-CD34 is a fluorescent cell counter through four optics (bright, yellow, red, green), which provides quick and convenient confirmation of Total CD45, Viable CD45, Viability of CD45, Total CD34, Viable CD34, Viability of CD34, Viable CD34 of Viable CD45, which are the main variables for determining stem cell extraction, storage and transplantation within up to 15 minutes. (Variable in different modes: 6 to 15 minutes). This device can be used in cell therapy center or clinics, laboratory or central lab in university, institute and hospital, blood bank and cord blood bank.

A convenient way of counting stem cells

The ADAMII-CD34 provies an accurate and precise results with rapid turnaround and minimal hands-on time by the operator. The high degree of precision of the system is due in part to the simplifying sample preparation steps and eliminating washing and reconstituting steps, which may cause cell loss and human error. In addition, results are reported using the test specific software, limiting interpretation steps by the user.

The stem cell therapy is carried out through adult stem cells, cord blood and clinical trials. A lot of research is going all around the world by medical professionals. The range of treats that stem cells is able to treat will be expanding more.

Therefore, NanoEntek has provided a variety of stem cell automated counters to support the development and productization of various stem cell. Research and development in stem cell will continue to expand and increase, so they can help to provide various equipment for quality control in stem cell therapy

Reference paper of using ADAM-CellT

[1] Single-Cell Analyses Identify Brain Mural Cells Expressing CD19 as Potential Off-Tumor Targets for CAR-T Immunotherapies, Cell, 183 (1), pp. 126-142.e17, October, 01, 2020.

Link: https://doi.org/10.1016/j.cell.2020.08.022

[2] Expression of the transcription factor PU.1 induces the generation of microglia-like cells in human cortical organoids, Nature Communications, 13, 430, 2022

Link: https://doi.org/10.1038/s41467-022-28043-y

[3] Heterozygous frameshift variants in HNRNPA2B1 cause early-onset oculopharyngeal muscular dystrophy, Nature Communications, 13, 2306, 2022

Link: https://doi.org/10.1038/s41467-022-30015-1

Reference paper of using ADAM II-CD34

[1] Enumeration of CD34 positive Stem Cells Using the ADAMII Image-based Fluorescence Cell Counter, Annals of Laboratory Medicine,

39(4), 388-395. 2019.

Link: https://doi.org/10.3343/alm.2019.39.4.388

[2] Clinical Applicability of Newly Developed Image-based Cell Counter for Counting CD34 + Cells: Comparison with Flow Cytometric Analysis,

Clinical Pediatric Hematology-Oncology, 23, pp. 125-132,2016

Link: http://doi.org/10.15264/cpho.2016.23.2.125

Reference

[1] Future of Cell Therapy in the Regenerative Medicine Market (2016), Frost&Sullivan

[2] Kashani Amr H., et al. "A bioengneered retinal pigment epthelial monolayer for advanced, dry age-related macular degeneration."Science translational medicine 10,435 (2018): eaao4097

Stem cell therapy

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