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ADAMII™ CD34

Fluorescence CD34 Counter

ADAMII™ CD34 is a bench top fluorescent CD34 counter that can be used in hematopoietic stem cell transplant for stem cell therapy.

KEY FEATURES & BENEFITS
- 40 minutes for counting CD34
- Easy to use
- Accurate and reliable result
- 4 channel (Bright, PerCP, PE, FITC)
- State-of-the-arts optics and image analysis software




ADAMII™ CD34, Fluorescence CD34 Counter 
ADAMII™ CD34 is a bench top fluorescent CD34 counter that can be used in hematopoietic stem cell transplant for stem cell therapy.

ADAMII™ CD34 started to release officially after receiving an approval from US FDA 510(k) on 08/09/2019 (FDA device listing Link)

ADVANTAGE #1

Revolutionary new way to count CD34!

ADAMII™ CD34 is an image-based fluorescence cell counter, which captures and analyzes multiple images.
It has 4-channel (Bright, Yellow (PE), Red (PerCP) and Green (FITC)) light sources.

Following specimens can be analyzed: normal and mobilized peripheral blood (PB/MPB),
as well as fresh hematopoietic progenitor cell-apheresis (HPC-A) collection.

ADAMII™ CD34 measures the percentage of viable CD34 positive cells from total leukocytes and absolute number of CD34 positive cells (cells/μL).





ADVANTAGE #2

Simple and easy to use

Add the sample and reagent to an empty tube, then add the lysis buffer.
Load the stained sample onto the slide and insert into the ADAMII™ CD34 instrument to get the result.

Up to 7 results will be generated and displayed : (1) Total CD45 (cells/μL) (2) Viable CD45 cells/μL (3) Total CD34 (%, cells/μL)
(4) Viable CD34 (%, cells/μL) (5) Viable CD34 of Viable CD45 (%) (6) CD45 Viability (%) (7) CD34 Viability (%).


ADAMII™ CD34 provides an accurate and precise results with rapid turnaround and minimal hands-on time by the operator.
The high degree of precision of the system is due in part to the simplifying sample preparation steps and
eliminating washing and reconstituting steps, which may cause cell loss and human error.
In addition, results are reported using the test specific software, limiting interpretation steps by the user.










ADVANTAGE #3

Substantially equivalent to flow cytometry

 ADAMII™ CD34 shows high correlation with flow cytometry along with low variation between sites, proving ADAMII™ CD34 provides high accuracy and consistency. 






 
Three replicates on two separate runs daily for 5 days were assessed for between days, runs, sites and total reproducibility %CV.




 




Reference paper of using ADAM II™ CD34 


1. Enumeration of CD34 positive Stem Cells Using the ADAMII Image-based Fluorescence Cell Counter, Annals of Laboratory Medicine, 39(4), 388-395. 2019.  


Link: https://synapse.koreamed.org/DOIx.php?id=10.3343/alm.2019.39.4.388



2. Clinical Applicability of Newly Developed Image-based Cell Counter for Counting CD34+ Cells: Comparison with Flow Cytometric Analysis, Clinical Pediatric Hematology-Oncology, 23 (2), pp. 125-132, 2016.


Link: http://www.cpho.or.kr/journal/view.html?vol=23&num=2&page=125&year=2016


  • Product name / Cat. No.

    ADAMII™ CD34 / ADAMII™

  • Light source

    1 brightfield, 3 fluorescences

  • Loading volume

    25 μL

  • Camera

    High–sensitivity monochrome CCD

  • Stage

    Automated X–Y–Z stage

  • Sample type

    HPC–A, Mobilized PB, Control, *Cord blood

  • Dimension

    300 x 420 x 370 mm (L x W x H)

  • Total frame

    75 frames

  • Weight

    19.3 kg

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