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ExTransfection™

Pipette-style Electroporation System

ExTransfection™ Electroporation System is a benchtop device that uses a single-chamber tip to deliver precise electrical pulses, enabling efficient transfection with high cell viability.


KEY FEATURES & BENEFITS

High-Efficiency Transfection and Superior Viability

- Applicable with Various Cell Types

- Consistent Electric Field

- Gold-coated electrodes

- Wide Range of Sample Sizes: 10 μL and 100 μL

*Available Worldwide (except in the USA)


 
ExTransfection™
Pipette-style Electroporation System


Product Overview
ExTransfection™ Electroporation System is an innovative, benchtop electroporation device designed for the efficient transfection of cell lines, primary cells, stem cells, and hematopoietic cells.
Utilizing ExTransfection™ Tip, a single electroporation chamber, it delivers precise electrical pulses, ensuring high transfection efficiency and cell viability by generating a strong, uniform electric field. 
Advantages and Features 

 High-Efficiency - Delivers intensive and even electrical pulses without unexpected loss 
 Superior Viability - Maintains high cell viability throughout the process
 Applicable - Compatible with various cell types, including mammalian, primary, stem, and blood cells
 Consistent - Ensures reproducible results under identical conditions
 Usability - Supports 10 μL and 100 μL sample volumes for different experimental needs
 Optimized Protocol - 300 cell-specific protocols available in the Protocol library

       






Protocol Library
ExTransfection™ offers a pre-programmed optimization protocol that helps an easy and quick optimization of electrical parameters for both adherent and suspended cells

Go to Library


Microporation Technology


NanoEntek is the original developer of microporation technology, designed to overcome the limitations of traditional electroporation methods using cuvettes. The core of microporation technology lies in its use of a capillary-type electric chamber with a minimal electrode surface area in direct contact with the sample. 


Small electrode surface area and increased gap between electrodes ensure uniform electric field within ExTransfection™ Tip. The insulated capillary minimizes electrolysis during electroporation, enhancing of transfection efficiency and cell viability. 


Comparison of ExTransfection™ Gold-Tip and Cuvette



 

 

High Transfection Efficiency


ExTransfection™ achieves high transfection efficiency in various cell lines, including primary cells.
The results are analyzed by fluorescence microscopy and flow cytometry. It consistently delivers successful transfection results across various primary cells.

      

Applications: Gene Editing, CRISPR/Cas9

CRISPR/Cas9 is a powerful genome editing tool that enables precise DNA modifications, offering potential cures for genetic diseases and advancements in personalized medicine.

Precise gene editing by CRISPR/Cas9 allows targeted correction of harmful mutations, transforming genetic disorder treatments.


ExTransfection™: Empowering Your CRISPR/Cas9 Research


ExTransfection is a highly efficient tool for gene editing, achieving a high gene knockout percentage and enabling important analyses such as double gene knockout, surface markers, transcription factors, and intracellular sensors.


Through this, it allows for precise analysis of gene functions in immune cells, making a significant contribution to immunological research and therapeutic development.

 



ExTransfection Specifications



 

Visit NanoEntek blog now!

Want to learn more about cell counting and cell therapy?

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FOR RESEARCH USE ONLY.
This product is not approved for diagnostic or therapeutic use.
 




 

  • Product name / Cat. No.

    ExTransfection™ Electroporation System / EXT1000

  • Reaction volume

    10 μL or 100 μL

  • Cell concentrations (Recommended)

    5 × 10E4–5 × 10E5 cells for 10 μL tip
    5 × 10E5–5 × 10E6 cells for 100 μL tip

  • Pulse voltage

    500–2,500 V

  • Pulse width

    1–100 ms

  • Number of pulses

    1–10

  • User interface

    8 inch touch screen

  • Electrical rating

    100–240 VAC, 4.0 A, 50/60 Hz

  • Electrical input

    24 VDC, 7.5 A, 180 W

  • Device weight

    6 Kg

  • Device dimensions

    251.6 (W) x 383.4 (D) x 185.1 (H) mm

ExTransfection™ Protocol Library

ExTransfection™ provides open protocols that make it easy for users to set up and run experiments. Its 24-well optimization method helps users to optimize transfection protocols easily and quickly.

ExTransfection™ also offers a pre-programmed optimization protocol that helps an easy and quick optimization of electrical parameters for both adherent and suspended cells.

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