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Dual-Florescence for Accurate Viability Analysis
AO (Acridine Orange) and DAPI (4′, 6-diamidino-2-phenylindole) are fluorescent dyes that bind to DNA,
staining the nucleus of targeted cells.
AO is a permeable dye that stains the nucleus of all cells, regardless of the cell’s condition.
DAPI is an impermeable dye that only stains the nucleus of non-viable cells with damaged membranes.
To accurately evaluate the viability of cell types like PBMCs, stem cells, and splenocytes, it is advisable to use dual-fluorescence staining.
This method effectively excludes non-nucleated cells (e.g., RBCs), platelets, and debris from the samples.
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AO/DAPI Staining Solution is to be used for EVE™ HT FL automated cell counter.
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FOR RESEARCH USE ONLY.
This product is not approved for diagnostic or therapeutic use.
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Product name / Cat. No
AO/DAPI Staining Solution / EVAD–960
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Staining method
AO/DAPI staining
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Quantity
20 mLⅹ2 bottles
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Storage
2 ℃–8 ℃
ExTransfection™ Protocol Library
ExTransfection™ provides open protocols that make it easy for users to set up and run experiments. Its 24-well optimization method helps users to optimize transfection protocols easily and quickly.
ExTransfection™ also offers a pre-programmed optimization protocol that helps an easy and quick optimization of electrical parameters for both adherent and suspended cells.
