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The World's Fastest Cell Counter
- Easy to use
- Automatically saved up to 500 results (email & USB available)
EVE™ Plus is the world's fastest automated cell counter. It is designed to measure cell count and viability with accuracy and precision by using the standard Trypan Blue and Erythrosin B solution within 1 second.
Cell counting operation
Auto focus & Manual focus
Auto focus
The system automatically displays total cells, live cells, dead cells, and viability percentage on the screen just 10 seconds after counting begins. Additional details, including cell concentration and size, are also provided.
Manual focus
After adjusting the image to your satisfaction using the ZOOM button, press the COUNT button to start counting.
Within 1 second, the total cells, live cells, dead cells, and viability percentage will appear on the screen.
Additional details, including cell concentration and size, are also provided.
Viability Measurement
Staining Method
EVE™ Plus is compatible with Trypan Blue (0.4%) and Erythrosin B (0.05%) staining, two of the most commonly used exclusion dyes for measuring cell viability. Both dyes share the same staining principle: viable cells with intact membranes exclude the dye, while non-viable cells are selectively stained.
Amid growing environmental and health concerns, the non-toxic Erythrosin B has gained popularity in many European countries and is increasingly recognized as a safer alternative to Trypan Blue for cell viability evaluation.
Total cell counting
Individual Counting of Clumped cells
Clumped cells were counted with EVE™ Plus, ADAM-MC2 (the nucleus cell counter), and 3 other manufacturers’ automated cell counters (A, B, and C). EVE™ Plus is comparable to the nucleus counting in terms of accuracy and precision across all cell lines.
In contrast, counters A, B, and C produced inaccurate counts when analyzing clumped cells.
EVE™ Plus effectively identifies and quantifies individual cells within the clumpy cells, ensuring reliable analysis.
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Performance data
Correlation Between Trypan Blue and Erythrosin B in EVE™ Plus
Jurkart cells were counted with EVE™ Plus (Trypan Blue, Erythrosin B) and ADAM™ MC2 (Acridine Orange (AO)•Propidium Iodide (PI)).
The result of EVE™ Plus using Trypan Blue and Erythrosin B showed a high correlation between the two dyes, and both also correlated well with the results from nucleus-based cell counter.
Correlation of EVE™ Plus and manual counting
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Product name / Cat. No.
EVE™ Plus / EVE–MC2
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Loading Volume
10 µL
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Measuring Volume
0.4 µL / test
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Measuring range
1 X 10E4–2 X 10E7 cell/mL
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Focus
Auto focusing & Manual focusing
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Display
LCD touch screen
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Weight
4 kg
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Size
274 x 274 x 333 mm
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Analysis Time
< 1 sec (Manual Focus), < 10 sec (Auto Focus)
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Dyes
Trypan blue stain, Erythrosine B stain
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Connectivity
WiFi (USB Dongle)
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Cell type
Cell line (clumpy cell, single cell)
Related downloads Total 3
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EVE PLUS_Brochure_NanoEntek
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EVE PLUS_Manual_NanoEntek
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EVE PLUS_Quick manual_NanoEntek
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